Readings and Resources on:

Monoclonal Antibodies

This web site serves as a gateway to recent articles, regulations and resources regarding production of monoclonal antibodies and its alternatives. We attempt to make a variety of resources on this issue more readily accessible by providing direct links to documents and websites on the internet.

Ascites-Associated Pain and Distress

ILAR Journal: 37(3) Adjuvants and Antibody Production
Altweb articles
Peterson, N.C.
"Behavioral, clinical, and physiologic analysis of mice used for ascites monoclonal antibody production."
Comp Med 50 (5) 2000:516-526.

In Vitro Alternatives

Pubmed Search 1
Search template on alternatives to ascites methods
Altweb Journals
Related articles on in vitro alternatives
Information resources for adjuvants and antibody production: Comparisons and alternative technologies, 1990-1998
AWIC Resource Series no. 3), edited by C.P. Smith, Animal Welfare Information Center, National Agricultural Library, Beltsville MD, USA 1997.
Contents: Overview of Adjuvants; Adjuvants Bibliography; ECVAM Workshop; Antibody Production Bibliography; Guidelines and Policies; Books and Proceedings; Companies and Institutes; Websites; Organizations
Marx, U.; Merz, W.
"In vivo and in vitro production of monoclonal antibodies: Bioreactors vs immune ascites,"
Chap.17, p.169-176, in Methods in Molecular Biology 45: Monoclonal antibody protocols, edited by W.C. Davis, Humana Press Inc., Totowa NJ, USA 1995.
McArdle, J.
"Alternatives to ascites production of monoclonal antibodies."
Animal Welfare Information Center: AWIC Newsletter 8(3-4) 1997/1998 Winter:1-4.
John McArdle (correspondence), Alternatives Research and Development Foundation, 14280 Golf View Dr, Eden Prairie MN 55346, USA, tel 612-949-2409, fax 612-949-2619
Peterson, N.C.
"Considerations for in vitro monoclonal antibody production."
Research in Immunology 149(6) 1998 Jul/Aug:553-557.

Polyclonal Antibody Production

Pubmed Search 2
Search template on polyclonal antibody as alternatives to ascites methods
ATLA and ALTEX Journals and Altweb articles
AWIC: Production Bibliography
Oogjes, G.
"Antibodies - rabbits to be replaced by hens?""
Animal Welfare Information Network 11996 March:4.

Adjuvants

ILAR Journal: 37(3) Adjuvants and Antibody Production
ATLA and ALTEX Journals and Altweb articles
AWIC: Adjuvants Bibliography
"COMPASS (COMparative Adjuvant Selection System)."
Animal Welfare Information Center: AWIC Newsletter 9(1-2) 1998 Fall:20.
Hanly, W.C.; Bennett, B.T.; Artwohl, J.E.
"Overview of adjuvants"
p. 1-8, in Information Resources for Adjuvants and Antibody Production: Comparisons and alternative technologies, 1990-1997, edited by C.P. Smith, Animal Welfare Information Center, National Agricultural Library, Beltsville MD, USA 1997.
Also published in BRL Bulletin 9(8/9) 1994 Aug/Sep:1-4
Leenaars, P.P.A.M.; Koedam, M.A.; Wester, P.W.; et al.
"Assessment of side effects induced by injection of different adjuvant/antigen combinations in rabbits and mice."
Laboratory Animals 32(4) 1998 Oct:387-406.
Nicklas, W. "
"Alternatives to Freund's adjuvant"
p. 1061-1064, in Animal Alternatives, Welfare and Ethics, edited by L.F.M. Van Zutphen and M. Balls, Elsevier Science, New York 1997.
Stewart-Tull, D.E.S.
"Adjuvant database."
European Adjuvant Group: EAG Letters 7(1) 1994:6.
Trouve, G.
"Testing for adjuvant safety."
European Adjuvant Group: EAG Letters 7(1) 1994:7-12.
Veterinary Public Health Inspectorate, Department of Animal Experimentation.
"Code of practice for the immunization of laboratory animals."
Rijswijk, The Netherlands 1993 Mar.
P De Greeve (correspondence), Chief Veterinary Public Health Inspectorate, PO Box 5406, 2280 HK Rijswijk, The Netherlands, tel 070 3407031, fax 070 3407080
Whary, M.T.; Carey, D.D.; Ferguson, F.G.
"Reduction in animal numbers required for antisera production using the subcutaneous chamber technique in rabbits."
Laboratory Animals 32(1) 1998 Jan:46-54.

Companies and Core Facilities

Selected List of Company and Institute Resources Providing New Technologies
Animal Welfare Information Center (AWIC), US Department of Agriculture, Agricultural Research Service, National Agricultural Library, Beltsville MD, USA.
Johns Hopkins University Cell Culture Laboratory (hybridoma growth)
Genetic Resources Core Facility, Johns Hopkins University, Baltimore MD USA 21202, tel 410-614-0060
National Cell Culture Center (purified monoclonal antibodies, 10 mg-100 g)
Mark Hirschel, PhD, Director, 8500 Evergreen Boulevard, Minneapolis MN, USA 55433, tel 800-325-1112, fax 612-786-0915, email ncccinfo@nccc.com
Serotec Antibody Location Service (free)
UK/international: 22 Bankside, Station Approach, Kidlington, Oxford OX5 1JE, tel 44-0-1865-852722, fax 44-0-1865-373899, email marketing@serotec.demon.co.uk
USA/Canada: 1017 Main Campus Drive, Suite 2450, NCSU, Raleigh NC 27606, tel 800-265-7376, fax 919-515-9980, email serotec@serotec-inc.com
Scandinavia: Postboks 62 Oppsal, N-0619, Oslo, Norway, tel 47-22-75-84-11, fax 47-22-75-84-13, email steph-ma@online.no
University of California, San Diego Core Cell Culture Facility
University of Pennsylvania Hybridoma Facility
Meenhard Herlyn, DVM, Director, Wistar Cancer Center Shared Resource Facilities, University of Pennsylvania, Philadelphia PA, USA 19104, tel 215-898-3950, email herlynm@wista.wistar.upenn.edu
University of Virginia Lymphocyte Culture Center
William Sutherland, PhD, Associate Director, Jordan Hall, Room 1060, Box 439, Health Sciences Center, University of Virginia, Charlottesville VA, USA 22906, tel 804-924-5379, fax 804-982-3912, email wms4f@unix.mail.virginia.edu

Resource Organizations

Alternatives to Animal Testing on the Web (Altweb): Special section on monoclonal antibodies
Animal Welfare Information Center (AWIC)
US Department of Agriculture, Agricultural Research Service, National Agricultural Library, 10301 Baltimore Ave, Beltsville, MD 20705, USA, tel 301-504-6212, email awic@nalusda.gov
European Adjuvant Group
Erik B. Lindblad (correspondence), Superfos Biosector a/s, PO Box 39, 2950 Vedbaek, Denmark, tel 42-89-31-11, fax 42-89-15-95
European Centre for the Validation of Alternative Methods (ECVAM)
Michael Balls (correspondence), JRC Environment Institute, 21020 Ispra (VA), Italy, tel 39-332-785996, fax 39-332-785336, email michael.balls@jrc.it
Foundation Research (Stiftung Forschung) 3R
Foundation Research 3R Projects on Monoclonal Antibodies
Postfach 1372, CH-3110, Munsingen, Switzerland, tel 41-31-722-08-30, fax 41-31-721-70-80
Institute for Laboratory Animal Research (ILAR)
National Research Council, 2101 Constitution Avenue, NW, Washington DC, USA 20418, tel 202-334-2590, fax 202-334-1687, email ILAR@nas.edu
Johns Hopkins Center for Alternatives to Animal Testing (CAAT)
111 Market Place, Suite 840, Baltimore, MD 21202-6709, USA, tel 410-223-1693, fax 410-223-1603, email CAAT@CAAT.spharbor.jhu.edu
Office for Protection from Research Risks (OPRR), Division of Animal Welfare
National Institutes of Health, 6100 Executive Blvd, Suite 3B01, MSC-7507, Rockville, MD 20892-7507, USA, tel 301-496-8101 x233, fax 301-402-0527, email CS19N@NIH.GOV

Bibliographies

Information resources for adjuvants and antibody production: Comparisons and alternative technologies, 1990-1998 (AWIC Resource Series no. 3)
edited by C.P. Smith, Animal Welfare Information Center, National Agricultural Library, Beltsville MD, USA 1997.
Contents: Overview of Adjuvants; Adjuvants Bibliography; ECVAM Workshop; Antibody Production Bibliography; Guidelines and Policies; Books and Proceedings; Companies and Institutes; Websites; Organizations

Policies and Recommendations

Altweb: Special Section on Monoclonal Antibodies - policies, regulations, guidelines, recommendations
Altweb: Special Section on Monoclonal Antibodies - reports and proceedings
AWIC: Guidelines and Policies
DePoyster, J.D. "Animal Care's position on Alternatives in Monoclonal Antibody Production
A workshop of The Johns Hopkins University Center for Alternatives to Animal Testing (CAAT) and The Office for Protection from Research Risks, National Institutes of Health, Baltimore MD, USA 1997 Sep 24-25.
European Biomedical Research Association – laboratory animal research legislation in:
Hendriksen, C.F.M. "A call for a European prohibition of monoclonal antibody production by the ascites procedure in laboratory animals"
Alternatives to Laboratory Animals: ATLA 26(4) 1998 Jul/Aug:523-536.
Hendriksen, C.; Rozing, J.; Van der Kamp, M.; et al. "The European perspective"
Proceedings for "Alternatives in Monoclonal Antibody Production", a workshop of The Johns Hopkins University Center for Alternatives to Animal Testing (CAAT) and The Office for Protection from Research Risks, National Institutes of Health, Baltimore MD, USA 1997 Sep 24-25.
Jackson, L.R.; Fox, J.G. "Institutional policies and guidelines on adjuvants and antibody production"
Institute of Laboratory Animal Resources: ILAR Journal 37(3) 1995:141-152.
Marx, U.; Embleton, M.J.; Fischer, R.; et al. "Monoclonal antibody production: The report and recommendations of ECVAM Workshop 23"
Alternatives to Laboratory Animals: ATLA 25(2) 1997:121-37.
Address for reprints: Reprints, European Centre for the Validation of Alternative Methods (ECVAM), TP580, JRC Environment Institute, 21020, Ispra (VA), Italy
National Health and Meidcal Research Council (NHMRC): Guidelines on Monoclonal Antibody Production
Sibal, L.R. "Regulatory issues regarding alternatives in monoclonal antibody production"
Proceedings for 'Alternatives in Monoclonal Antibody Production', a workshop of The Johns Hopkins University Center for Alternatives to Animal Testing (CAAT) and The Office for Protection from Research Risks, National Institutes of Health, Baltimore MD, USA 1997 Sep 24-25.
Ward, P.A.; Adams, J.; Faustman, D.; et al. "Monoclonal Antibody Production: A Report of the Committee on Methods of Producing Monoclonal Antibodies
Institute for Laboratory Animal Research (ILAR), National Research Council, National Academy Press, Washington, DC 1999.

Institutional Animal Care and Use Guidelines

Guidelines for using the ascites method of monoclonal antibody production
Johns Hopkins Animal Care and Use 2001 September 20.
IACUC/LARC standard procedures on antibody production.
University of California, San Francisco.
Recommendations for the production of ascitic fluid
University of Iowa, Animal Care Unit 1998 Feb 25.
Recommendations for the production of hybridomas
University of Iowa, Animal Care Unit 1997 Jun 17.
Recommendations for use and alternatives to Freund's Complete Adjuvant.
University of Iowa, Animal Care Unit 1996 Nov 19.
Monoclonal Antibody Production Guidelines
Institutional Animal Care and Use Committee, University of Tennessee.

News and Issues

Altweb News on Monoclonal Antibody
Lamborn, C. "Critics cry foul in MAb review."
Lab Animal 28(1) 1999 Jan:10-11.
Lamborn, C. "NRC Report opposes ban on MAb ascites production."
Lab Animal 28(5) 1999 May:10-11.
Matfield, M. "ECVAM report: monoclonal Ab production."
European Biomedical Research Association: EBRA Bulletin 1997 Aug.
Mark Matfield (correspondence), European Biomedical Research Association, 58 Great Marlborough St, London W1V 1DD, UK, email secretariat@ebra.org
McArdle, J. "Antibodies without animals."
PSYETA News 18 1998 Spring:6-7.
Schulhof, J.; Lamborn, C. "Jumping from the banned wagon."
Lab Animal 27(2) 1998 Feb:9
"US campaign to end ascites methods."
Fund for the Replacement of Animals in Medical Experiments: FRAME News 43 1997 Sep:8.

Mary W. Wood, MLS — Librarian, UCCAA

Contributors:
Hsin-Yi Weng, DVM, MPH
Caroline Crispino, MS